Adult Male Syrian Hamsters (Mesocricetus auratus) Exhibit Daily Oscillations in Their Serum Levels of Melatonin and Leptin As Well As in the Expression of the GnRH, GnIH, and Kisspeptin Genes

The complex neuronal and hormonal interaction between the brain gonads controls neuroendocrine reproductive axis. GnRH, GnIH, kisspeptin are important neuropeptides in this relationship. Although seasonal variations of these have been demonstrated photoperiodic animals, there is no clear evidence their daily rhythms. Melatonin leptin hormones also two regulation. In our study, relationship melatonin with rhythm gene expressions protein oscillations was examined. Adult male Syrian hamsters were exposed to long photoperiod at end 30-day experimental period, blood tissue samples from each group collected 04:00 h, 12:00 20:00 00:00 h. Daily rhythms determined by ELISA. Quantitative analysis Kisspeptin, β-actin genes performed corresponding primers Real-Time PCR. Protein Western Blot technique. Serum levels showed an inverse rhythmic Leptin level found be low while high dark. observed mRNA oscillations. As a result, findings could imply that all relationships melatonin, leptin, not reproductive, but rather metabolic nature.